{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Tecan Infinite 200 PRO\n",
    "\n",
    "The Tecan Infinite 200 PRO is a multimode microplate reader that supports absorbance, fluorescence, and luminescence measurements. This backend targets the Infinite \"M\" series (e.g., Infinite 200 PRO M Plex)."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": "from pylabrobot.plate_reading import PlateReader\nfrom pylabrobot.plate_reading.tecan import ExperimentalTecanInfinite200ProBackend"
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": "pr = PlateReader(name=\"PR\", size_x=0, size_y=0, size_z=0, backend=ExperimentalTecanInfinite200ProBackend())\nawait pr.setup()"
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "await pr.open()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Before closing, assign a plate to the plate reader. This determines the well positions for measurements."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "from pylabrobot.resources import Cor_96_wellplate_360ul_Fb\n",
    "plate = Cor_96_wellplate_360ul_Fb(name=\"plate\")\n",
    "pr.assign_child_resource(plate)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "await pr.close()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Absorbance\n",
    "\n",
    "Read absorbance at a specified wavelength (230-1000 nm)."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "import matplotlib.pyplot as plt\n",
    "\n",
    "data = await pr.read_absorbance(wavelength=450)\n",
    "plt.imshow(data)\n",
    "plt.colorbar(label=\"OD\")\n",
    "plt.title(\"Absorbance at 450 nm\")"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": "## Fluorescence\n\nRead fluorescence with specified excitation and emission wavelengths (230-850 nm). The focal height is in millimeters."
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": "data = await pr.read_fluorescence(\n    excitation_wavelength=485,\n    emission_wavelength=528,\n    focal_height=20.0\n)\nplt.imshow(data)\nplt.colorbar(label=\"RFU\")\nplt.title(\"Fluorescence (Ex: 485 nm, Em: 528 nm)\")"
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": "## Luminescence\n\nRead luminescence. The focal height is in millimeters."
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": "data = await pr.read_luminescence(focal_height=20.0)\nplt.imshow(data)\nplt.colorbar(label=\"RLU\")\nplt.title(\"Luminescence\")"
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Reading specific wells\n",
    "\n",
    "You can specify a subset of wells to read instead of the entire plate."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "wells = plate.get_items([\"A1\", \"A2\", \"B1\", \"B2\"])\n",
    "data = await pr.read_absorbance(wavelength=450, wells=wells)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## Cleanup"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "await pr.stop()"
   ]
  }
 ],
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