pylabrobot.agilent.biotek.el406.plate_washer.PlateWasher.prime

async PlateWasher.prime(volume: float = 10000.0, buffer: Literal['A', 'B', 'C', 'D'] = 'A', flow_rate: int = 9, low_flow_volume: float = 5000.0, submerge_duration: float = 0.0) → None

Prime the manifold fluid lines.

Fills the wash manifold tubing with liquid from the specified buffer. This is typically done at the start of a protocol to ensure the lines are filled and ready for dispensing.

Parameters:

• volume (float) – Prime volume in uL (5000-999000). Wire resolution: 1000 uL.

• buffer (Literal['A', 'B', 'C', 'D']) – Buffer valve selection (A, B, C, D).

• flow_rate (int) – Flow rate (3-11).

• low_flow_volume (float) – Low flow path volume in uL (5000-999000). Set to 0 to disable.

• submerge_duration (float) – Submerge duration in seconds (0 to disable, 60-86340). Must be a multiple of 60.

Raises:

ValueError – If parameters are invalid.

Return type:

None