pylabrobot.molecular_devices.spectramax.SpectraMaxM5.read_time_resolved_fluorescence

async SpectraMaxM5.read_time_resolved_fluorescence(plate: Plate, excitation_wavelengths: List[int], emission_wavelengths: List[int], cutoff_filters: List[int], delay_time: int, integration_time: int, read_type: Literal['endpoint', 'kinetic', 'spectrum', 'well_scan'] = 'endpoint', read_order: Literal['column', 'wavelength'] = 'column', calibrate: Literal['on', 'once', 'off'] = 'once', shake_settings: ShakeSettings | None = None, carriage_speed: Literal['normal', 'slow'] = 'normal', read_from_bottom: bool = False, pmt_gain: Literal['auto', 'high', 'medium', 'low'] | int = 'auto', flashes_per_well: int = 50, kinetic_settings: KineticSettings | None = None, spectrum_settings: SpectrumSettings | None = None, cuvette: bool = False, settling_time: int = 0, timeout: int = 600) → List[Dict]

Read time-resolved fluorescence.

Parameters:

• plate (Plate)

• excitation_wavelengths (List[int])

• emission_wavelengths (List[int])

• cutoff_filters (List[int])

• delay_time (int)

• integration_time (int)

• read_type (Literal['endpoint', 'kinetic', 'spectrum', 'well_scan'])

• read_order (Literal['column', 'wavelength'])

• calibrate (Literal['on', 'once', 'off'])

• shake_settings (ShakeSettings | None)

• carriage_speed (Literal['normal', 'slow'])

• read_from_bottom (bool)

• pmt_gain (Literal['auto', 'high', 'medium', 'low'] | int)

• flashes_per_well (int)

• kinetic_settings (KineticSettings | None)

• spectrum_settings (SpectrumSettings | None)

• cuvette (bool)

• settling_time (int)

• timeout (int)

Return type:

List[Dict]