pylabrobot.thermo_fisher.kingfisher.bdz.MixStep#

class pylabrobot.thermo_fisher.kingfisher.bdz.MixStep(name: str, shake_duration: int = 30, shake_speed: str = '563b24fa-2eb7-4497-928b-5e91b740a01e', loop_count: int = 1, pause_tip_position: str = 'AboveSurface', release_beads_enabled: bool = True, release_beads_duration: int = 0, release_beads_speed: str = '6e89445e-98b2-43c5-8ae5-c37ed517f506', collect_beads_enabled: bool = True, collect_count: int = 3, collect_time: int = 1, heating_enabled: bool = False, temperature: int = 37, preheat: bool = False, pause_enabled: bool = False, pause_message: str = '', postmix_enabled: bool = False, postmix_duration: int = 0, postmix_speed: str = '563b24fa-2eb7-4497-928b-5e91b740a01e', post_temperature_enabled: bool = False, post_temperature: int = 10, sample_plate_index: int = 0, well_group_name: str = 'Plate')#

Bases: object

A Mix step — the primary step type in KingFisher protocols.

The instrument shakes a sample plate against a tip plate, optionally releasing beads beforehand and collecting them afterwards.

well_group_name controls which well group the step addresses: "Plate" = whole-plate (Presto always; Duo whole-plate steps). Row letter "A"–"H" = row-targeted step (Duo only).

Attributes

Methods

__init__(name[, shake_duration, ...])

Parameters:

name (str)
shake_duration (int)
shake_speed (str)
loop_count (int)
pause_tip_position (str)
release_beads_enabled (bool)
release_beads_duration (int)
release_beads_speed (str)
collect_beads_enabled (bool)
collect_count (int)
collect_time (int)
heating_enabled (bool)
temperature (int)
preheat (bool)
pause_enabled (bool)
pause_message (str)
postmix_enabled (bool)
postmix_duration (int)
postmix_speed (str)
post_temperature_enabled (bool)
post_temperature (int)
sample_plate_index (int)
well_group_name (str)

`collect_beads_enabled`
`collect_count`
`collect_time`
`heating_enabled`
`loop_count`
`pause_enabled`
`pause_message`
`pause_tip_position`
`post_temperature`
`post_temperature_enabled`
`postmix_duration`
`postmix_enabled`
`postmix_speed`
`preheat`
`release_beads_duration`
`release_beads_enabled`
`release_beads_speed`
`sample_plate_index`
`shake_duration`
`shake_speed`
`temperature`
`well_group_name`
`name`