pylabrobot.bmg_labtech.vantastar.driver.master.VANTAstarDriver.build_absorbance_run#

VANTAstarDriver.build_absorbance_run(wavelengths: Sequence[float], *, flashes: int, settling_time: float, geometry: PlateGeometry = PlateGeometry(length=127.76, width=85.48, first_x=14.38, first_y=11.24, last_x=113.38, last_y=74.24, columns=12, rows=8, well_size=6.58, well_shape=0), well_mask: bytes | None = None, well_scan: Literal['point', 'orbital', 'spiral', 'matrix'] = 'point', scan_diameter: int = 3, corner: Literal['top_left', 'top_right', 'bottom_left', 'bottom_right'] = 'top_left', reading_direction: Literal['column_major', 'row_major'] = 'row_major', bidirectional: bool = True) → bytes#

RUN fields for an absorbance endpoint read (MeasurementMode 2).

Besides the wavelengths the reader reads two reference wavelengths, 900 and 993 nm (Voyager’s pathlength-correction pair), and a reference channel.

Parameters:
  • wavelengths (Sequence[float]) – wavelengths in nm (0.1 nm resolution).

  • flashes (int) – flashes per well.

  • settling_time (float) – seconds to settle before reading each well (20 ms steps).

  • geometry (PlateGeometry) – the plate.

  • well_mask (bytes | None) – the wells to read (encode_well_mask); None reads every well.

  • well_scan (Literal['point', 'orbital', 'spiral', 'matrix']) – point, orbital or spiral (encode_well_scan); scan_diameter: its diameter, mm.

  • corner (Literal['top_left', 'top_right', 'bottom_left', 'bottom_right']) – the reading order (encode_reading_mode).

  • reading_direction (Literal['column_major', 'row_major']) – the reading order (encode_reading_mode).

  • bidirectional (bool) – the reading order (encode_reading_mode).

  • scan_diameter (int)

Return type:

bytes