Byonoy Luminescence 96 Automate#

  • OEM Link

  • Communication Protocol / Hardware: HID / USB-A/C

  • Communication Level: Firmware

  • VID:PID 16d0:119b

  • Takes a single SLAS-format 96-wellplate on the base unit, enables movement of the reader unit over it, and reads all 96 wells simultaneously.

Setup Instructions (Physical)#

The Byonoy Luminescence 96 Automate (L96A) is a luminescence plate reader consisting of…

  1. a base_unit which holds the plate,

  2. a reader_unit containing the light sensors that sits on top of the base unit.

Communication#

It requires only one cable connection to be operational:

  1. USB cable (USB-C at reader_unit end; USB-A at control PC end)

Setup Instructions (Programmatic)#

%load_ext autoreload
%autoreload 2

from pylabrobot.plate_reading.byonoy import byonoy_l96a

base_unit, reader_unit = byonoy_l96a("Luminescence96 Automate")
reader_unit

ByonoyLuminescence96Automate(name='Luminescence96 Automate_reader', location=Coordinate(000.000, 000.000, 006.300), size_x=138, size_y=97.5, size_z=41.7, category=plate_reader)

await reader_unit.setup()

Measure Luminescence#

Before you can do a plate reading measurement in PLR, you need to assign a plate to the reader.

from pylabrobot.resources.corning.plates import Cor_96_wellplate_360ul_Fb
demo_plate = Cor_96_wellplate_360ul_Fb(name="plate")

reader_unit.assign_child_resource(demo_plate)

data = await reader_unit.read_luminescence(
  focal_height=1,
  use_new_return_type=True,
)

print(f"Time: {data[0]['time']}")
print(f"Temperature: {data[0]['temperature']}")
print("Data")
print(data[0]['data'])

Time: 1770236622.314785
Temperature: None
Data
[[200552272.0, 201907664.0, 218108208.0, 234161952.0, 251950976.0, 8104904.0, 1781678.0, 1690456.0, 2708988.0, 2377269.0, 5855379.0, 289570432.0], [183959648.0, 183223568.0, 186845840.0, 187736528.0, 3184948.0, 1934996.0, 1431882.0, 1225921.0, 1186311.0, 1238371.0, 1392693.0, 3190326.0], [209724544.0, 200640128.0, 4695649.0, 2133116.0, 2067423.0, 1974764.0, 1769515.0, 1576723.0, 1396270.0, 1392762.0, 1163494.0, 1340111.0], [8631671.0, 2948252.0, 1878185.0, 1944314.0, 1928056.0, 1818789.0, 1538142.0, 1391728.0, 1203908.0, 1180876.0, 1094184.0, 1385255.0], [2561222.0, 2007909.0, 2202352.0, 2187162.0, 2016595.0, 1628429.0, 1532333.0, 1342909.0, 1310737.0, 1187646.0, 1123093.0, 1284595.0], [2612154.0, 2109698.0, 2374385.0, 2376566.0, 2073923.0, 1862185.0, 1761917.0, 1637089.0, 1429714.0, 1279758.0, 937221.0, 1471533.0], [3401529.0, 2545716.0, 2582033.0, 2696296.0, 2458846.0, 2448346.0, 2106862.0, 1956427.0, 1705456.0, 1578239.0, 1126263.0, 1378166.0], [6511548.0, 5467399.0, 6249315.0, 6408278.0, 5996644.0, 5163665.0, 5879837.0, 5222682.0, 4798715.0, 4238464.0, 3052718.0, 1889772.0]]

Disconnect from Reader#

await reader_unit.stop()

Resource model#

In the example above, we instantiated the Byonoy Luminescence 96 Automate reader using the byonoy_l96a function, which automatically creates the necessary resources for the reader.

base_unit.reader_unit_holder.resource is reader_unit

True