pylabrobot.liquid_handling.liquid_handler.LiquidHandler.aspirate#

async LiquidHandler.aspirate(resources: Sequence[Container], vols: List[float], use_channels: List[int] | None = None, flow_rates: List[float | None] | None = None, offsets: List[Coordinate] | None = None, liquid_height: List[float | None] | None = None, blow_out_air_volume: List[float | None] | None = None, **backend_kwargs)#

Aspirate liquid from the specified wells.

Examples

Aspirate a constant amount of liquid from the first column:

>>> await lh.aspirate(plate["A1:H1"], 50)

Aspirate an linearly increasing amount of liquid from the first column:

>>> await lh.aspirate(plate["A1:H1"], range(0, 500, 50))

Aspirate arbitrary amounts of liquid from the first column:

>>> await lh.aspirate(plate["A1:H1"], [0, 40, 10, 50, 100, 200, 300, 400])

Aspirate liquid from wells in different plates:

>>> await lh.aspirate(plate["A1"] + plate2["A1"] + plate3["A1"], 50)

Aspirating with a 10mm z-offset:

>>> await lh.aspirate(plate["A1"], vols=50, offsets=[Coordinate(0, 0, 10)])

Aspirate from a blue bucket (big container), with the first 4 channels (which will be spaced equally apart):

>>> await lh.aspirate(blue_bucket, vols=50, use_channels=[0, 1, 2, 3])
Parameters:

  • resources (Sequence[Container]) – A list of wells to aspirate liquid from. Can be a single resource, or a list of resources. If a single resource is specified, all channels will aspirate from the same resource.

  • vols (List[float]) – A list of volumes to aspirate, one for each channel. If vols is a single number, then all channels will aspirate that volume.

  • use_channels (List[int] | None) – List of channels to use. Index from front to back. If None, the first len(wells) channels will be used.

  • flow_rates (List[float | None] | None) – the aspiration speed. In ul/s. If None, the backend default will be used.

  • offsets (List[Coordinate] | None) – List of offsets for each channel, a translation that will be applied to the aspiration location.

  • liquid_height (List[float | None] | None) – The height of the liquid in the well wrt the bottom, in mm.

  • blow_out_air_volume (List[float | None] | None) – The volume of air to aspirate after the liquid, in ul. If None, the backend default will be used.

  • backend_kwargs – Additional keyword arguments for the backend, optional.

Raises: