pylabrobot.thermocycling.opentrons_backend.OpentronsThermocyclerBackend#

class pylabrobot.thermocycling.opentrons_backend.OpentronsThermocyclerBackend(opentrons_id: str)#

HTTP-API backend for the Opentrons GEN-1/GEN-2 Thermocycler.

All core functions are supported. run_profile() is fire-and-forget, since PCR runs can outlive the decorator’s default timeout.

Methods

`__init__`(opentrons_id)	Create a new backend bound to a specific thermocycler.
`close_lid`()	Close the thermocycler lid.
`deactivate_block`()	Deactivate the block heater.
`deactivate_lid`()	Deactivate the lid heater.
`deserialize`(data)
`get_all_instances`()
`get_block_current_temperature`()	Get the current block temperature in °C.
`get_block_target_temperature`()	Get the block target temperature in °C.
`get_current_cycle_index`()	Get the zero-based index of the current cycle from the Opentrons API.
`get_current_step_index`()	Get the zero-based index of the current step from the Opentrons API.
`get_hold_time`()	Get remaining hold time in seconds.
`get_lid_current_temperature`()	Get the current lid temperature in °C.
`get_lid_status`()	Get the lid open/closed status.
`get_lid_target_temperature`()	Get the lid target temperature in °C.
`get_total_cycle_count`()	Get the total cycle count.
`get_total_step_count`()	Get the total number of steps in the current cycle.
`open_lid`()	Open the thermocycler lid.
`run_profile`(profile, block_max_volume)	Enqueue and return immediately (no wait) the PCR profile command.
`serialize`()	Include the Opentrons module ID in serialized state.
`set_block_temperature`(temperature)	Set block temperature in °C.
`set_lid_temperature`(temperature)	Set lid temperature in °C.
`setup`()	No extra setup needed for HTTP-API thermocycler.
`stop`()	Gracefully deactivate both heaters.