pylabrobot.plate_reading.biotek_backend.Cytation5Backend.capture#

async Cytation5Backend.capture(row: int, column: int, mode: ImagingMode, objective: Objective, exposure_time: float | Literal['auto'], focal_height: float | Literal['auto'], gain: float | Literal['auto'], plate: Plate, led_intensity: int = 10, coverage: Literal['full'] | Tuple[int, int] = (1, 1), center_position: Tuple[float, float] | None = None, overlap: float | None = None, color_processing_algorithm: int = -1, pixel_format: int = -1) → List[List[List[float]]]#

Capture image using the microscope

speed: 211 ms ± 331 μs per loop (mean ± std. dev. of 7 runs, 10 loops each)

Parameters:

  • exposure_time (float | Literal['auto']) – exposure time in ms, or "auto"

  • focal_height (float | Literal['auto']) – focal height in mm, or "auto"

  • coverage (Literal['full'] | ~typing.Tuple[int, int]) – coverage of the well, either "full" or a tuple of (num_rows, num_columns). Around center_position.

  • center_position (Tuple[float, float] | None) – center position of the well, in mm from the center of the selected well. If None, the center of the selected well is used (eg (0, 0) offset). If coverage is specified, this is the center of the coverage area.

  • color_processing_algorithm (int) – color processing algorithm. See PySpin.SPINNAKER_COLOR_PROCESSING_ALGORITHM_*

  • pixel_format (int) – pixel format. See PySpin.PixelFormat_*

  • row (int)

  • column (int)

  • mode (ImagingMode)

  • objective (Objective)

  • gain (float | Literal['auto'])

  • plate (Plate)

  • led_intensity (int)

  • overlap (float | None)

Return type:

List[List[List[float]]]