pylabrobot.plate_reading.biotek_backend.Cytation5Backend

class pylabrobot.plate_reading.biotek_backend.Cytation5Backend(timeout: float = 20, camera_serial_number: float | None = None)

Bases: ImageReaderBackend

Backend for biotek cytation 5 image reader.

For imaging, the filter used during development is Olympus 4X PL FL Phase, and the magnification is 4X, numerical aperture is 0.13. The camera is interfaced using the Spinnaker SDK, and the camera used during development is the Point Grey Research Inc. Blackfly BFLY-U3-23S6M.

Methods

__init__([timeout, camera_serial_number])
auto_focus([timeout])
capture(row, column, mode, exposure_time, ...)	Capture image using the microscope
close()	Close the plate reader.
deserialize(data)
get_current_temperature()	Get current temperature in degrees Celsius.
get_firmware_version()
get_serial_number()
led_off()
led_on([intensity])
open()	Open the plate reader.
read_absorbance(wavelength)	Read the absorbance from the plate reader.
read_fluorescence(excitation_wavelength, ...)	Read the fluorescence from the plate reader.
read_luminescence(focal_height)	Read the luminescence from the plate reader.
select(row, column)
send_command(command[, parameter, ...])
serialize()
set_auto_exposure(auto_exposure)
set_exposure(exposure)	exposure (integration time) in ms, or "auto"
set_focus(focal_position)	focus position in mm
set_gain(gain)	gain of unknown units, or "auto"
set_imaging_mode(mode)
setup([use_cam])	Set up the plate reader.
shake(shake_type)	Warning: the duration for shaking has to be specified on the machine, and the maximum is 16 minutes.
stop()	Close all connections to the plate reader and make sure setup() can be called again.
stop_shaking()

Parameters:

• timeout (float)

• camera_serial_number (float | None)