pylabrobot.liquid_handling.backends.hamilton.STAR.STAR.pierce_foil#

async STAR.pierce_foil(wells: Well | List[Well], piercing_channels: List[int], hold_down_channels: List[int], move_inwards: float, spread: Literal['wide', 'tight'] = 'wide', one_by_one: bool = False)#

Pierce the foil of the media source plate at the specified column. Throw away the tips after piercing because there will be a bit of foil stuck to the tips. Use this method before aspirating from a foil-sealed plate to make sure the tips are clean and the aspirations are accurate.

Parameters:

  • wells (Well | List[Well]) – Well or wells in the plate to pierce the foil. If multiple wells, they must be on one column.

  • piercing_channels (List[int]) – The channels to use for piercing the foil.

  • hold_down_channels (List[int]) – The channels to use for holding down the plate when moving up the piercing channels.

  • spread (Literal['wide', 'tight']) – The spread of the piercing channels in the well.

  • one_by_one (bool) – If True, the channels will pierce the foil one by one. If False, all channels will pierce the foil simultaneously.

  • move_inwards (float)